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puc19 t7 3wjdb t plasmid  (Addgene inc)


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    Structured Review

    Addgene inc puc19 t7 3wjdb t plasmid
    Puc19 T7 3wjdb T Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc19+t7+3wjdb+t+plasmid/pUC19-T7-3WJdB-T+(Plasmid+%2387308)/us12203144-726-8-10
    Average 91 stars, based on 3 article reviews
    puc19 t7 3wjdb t plasmid - by Bioz Stars, 2026-11
    91/100 stars

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    Plasmid Preparation:

    Article Title: Analytes' detection using regulated in vitro transcription
    Article Snippet: Oligonucleotides for cloning, amplification, and transcription were ordered from Integrated DNA Technologies and resuspended in H2O at 100 μM. .. For unregulated transcription of the 3WJdB aptamer, the pUC19-T7-3WJdB-T plasmid (Addgene plasmid #87308) was used.19 Templates for regulated transcription of the 3WJdB aptamer were cloned into pUC19 plasmids by Gibson assembly. .. Assembled plasmids were heat-shock transformed into NEB Turbo Competent E. coli cells (New England Biolabs) and clonally isolated by streaking onto antibiotic-selective LB-agar plates grown overnight at 37° C. Plasmids were then purified using a QIAprep miniprep kit (Qiagen) following the manufacturer's protocol and verified by Sanger sequencing at Quintara Biosciences.

    Clone Assay:

    Article Title: Analytes' detection using regulated in vitro transcription
    Article Snippet: Oligonucleotides for cloning, amplification, and transcription were ordered from Integrated DNA Technologies and resuspended in H2O at 100 μM. .. For unregulated transcription of the 3WJdB aptamer, the pUC19-T7-3WJdB-T plasmid (Addgene plasmid #87308) was used.19 Templates for regulated transcription of the 3WJdB aptamer were cloned into pUC19 plasmids by Gibson assembly. .. Assembled plasmids were heat-shock transformed into NEB Turbo Competent E. coli cells (New England Biolabs) and clonally isolated by streaking onto antibiotic-selective LB-agar plates grown overnight at 37° C. Plasmids were then purified using a QIAprep miniprep kit (Qiagen) following the manufacturer's protocol and verified by Sanger sequencing at Quintara Biosciences.



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